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Methyl-beta-cyclodextrin (MBCD) can improve the efficiency of sperm DNA uptake

Methyl-beta-cyclodextrin (MBCD, CAS No.128446-36-6) is a water-soluble cyclic oligosaccharide. Studies have shown that Methyl-β-cyclodextrin can remove cholesterol from the sperm plasma membrane, thereby increasing the absorption of DNA by the sperm, and therefore fertilizing the sperm and developing embryos. And mouse sperm capacitation has beneficial effects. Experiments have used different concentrations of MBCD to treat fresh sperm and monitor the direct effect of methyl betadex on sperm parameters, confirming this possibility.

Sperm can be used as a natural carrier for the production of transgenic animals. In the study on the applicability of Methyl-beta-cyclodextrin in rooster SMGT, the experimenters transfected foreign DNA into rooster sperm cells and used different concentrations of Methyl-beta-cyclodextrin to conduct comparative experiments to evaluate and determine the efficiency and effectiveness of Methyl-beta-cyclodextrin on DNA absorption after transfection. The quality of sperm is affected, and then the transfected sperm is used for artificial insemination to produce transgenic chickens.

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